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  1. Products
  2. qPCR & PCR
  3. Custom primers
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Custom primers

Primer design is key for generating high-quality PCR results. IDT offers a variety of custom primers and easy-to-use primer design tools to help ensure that you generate the primers you need to drive your research forward.

Design tools

Create sequences and submit requests using one of our free, online design tools. These tools are powered by design engines based on sophisticated algorithms devised considering nearest neighbor analysis and other sequence design best practices.

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PrimeTime™ qPCR Primer Assays

PrimeTime qPCR Primer Assays for use with intercalating dyes, such as SYBR® Green dyes*, include predesigned primer sets for human, mouse, or rat sequences. Custom assays may be created for all other species using the PrimerQuest™ Tool. Predesigned and custom assays can be provided in tubes or plates.

* SYBR is a trademark of Thermo Fisher Scientific

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RxnReady™ Primer Pools

RxnReady Primer Pools are two premixed, custom DNA oligos delivered in a single tube. A variety of customization options are available: incorporate modifications or mixed bases into sequence designs, select a specific purification technique, and request final formulation of the pools.

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rhPCR primers (for RNase H-dependent PCR)

rhPCR is an advanced nucleic acid amplification technology that diminishes negative primer-dimer effects. Use rhPCR primers (rhPrimers) to create rhPCR assays for custom applications requiring increased precision and higher signal. These primers include an RNA base and 3' blocking moiety, which, when used in combination with RNase H2 enzyme, comprise the components needed to perform rhPCR.

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Oligo modifications

Choose from a variety of modifications and mixed bases that can be incorporated at 5', internal, or 3' positions of primers or probes to improve PCR analysis in regions of complexity or be incorporated into oligonucleotides designed for custom applications.

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GMP and OEM services

IDT offers specialized manufacturing services for GMP* and OEM business partners. Review our capabilities and see how we can best support your clinical diagnostics, resale, or commercial oligo needs.

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Overview

The design and synthesis of a primer pair are important considerations when generating PCR or qPCR data. Poor design choices, erroneous or truncated sequences, and ineffective purification can lead to unusable results. We have designed a suite of online tools to facilitate the intelligent design of your primers. We also help you generate consistent, quality data by providing primers in a variety of formats that have been synthesized on our state-of-the-art oligonucleotide synthesis platforms.

Primer-only qPCR experiments offer a cost- and time-saving approach to qPCR as probe design is not necessary and intercalating dyes are budget-friendly for large-scale experiments. In primer-only qPCR assays, like the PrimeTime qPCR Primer Assays, a DNA polymerase extends from the primers and an intercalating dye attaches to the double-stranded regions creating a fluorescent signal. Because primer-only qPCR experiments can identify amplification products from non-specific targets or primer-dimers, IDT also offers rhPCR primers that have a 3' blocking group that needs to be removed by RNase H2 before amplification. RNase H2 is sensitive to mismatches around the cleavage site, and therefore, reduces the amount of non-specific target or primer-dimer amplification. Additionally, IDT offers probe-based qPCR reagents that reduce the amount of non-specific amplification.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

GMP refers to products manufactured under ISO 13485: 2016 QMS. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations for their legal marketing.

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