DsiRNAs are chemically synthesized, 27 nt RNA duplexes that are optimized for Dicer processing and are ideal for small-scale in vitro applications. These 27mer duplexes have increased potency in RNAi compared to traditional 21mer siRNAs. DsiRNAs were originally developed as a collaborative effort with Dr John Rossi of the Beckman Research Institute of the City of Hope (Duarte, CA, USA). Updated design rules that have been developed at IDT have resulted in potent DsiRNAs that are available only from IDT. Each DsiRNA is purified and identified by ESI mass spectrometry*. Unless otherwise noted, DsiRNAs are provided dry in tubes. All QC data is provided free of charge on our website.
For ultimate convenience, you can acquire all the necessary reagents for RNA knockdown by ordering your DsiRNAs and controls in a TriFECTa RNAi Kit.
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.