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PrimerQuest™ Tool

Easily design primers or assays for PCR, qPCR, or sequencing (any species).

Customization of ~45 parameters, allowing qPCR assay designs:

With specific primer, probe, or amplicon criteria
Across a specified location
Design algorithm includes multiple checks to reduce primer-dimer formation

Design algorithm includes multiple checks to reduce primer-dimer formation
Provides flexible sequence entry and batch entries (up to 50 sequences)

Research-friendly UX

Flexible input and advanced parameters to optimize your custom order:

Enter your sequence
Adjust calculation options
Choose a function

New to the PrimerQuest Tool?

Our video will introduce you to the basics and get you up and running quickly. We’ll go over the various functionalities available in the tool, using example sequences.

The power to customize 45 different parameters

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Enter your sequence

Manually using FASTA format
Download using Genbank or Accession ID
Upload an Excel file

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Design and customize

PCR (2 primers)
qPCR (2 primers + probe; for use in 5' nuclease assays)
qPCR (2 primers; for use with intercalataing dyes)
Customize design parameters (optional)

Icons Library_Sky_85x85_How to order

Select and order assays

Click the box(es) next to the set(s) you wish to order.
Click the green assay bar(s) in the schematic.
For batch analyses, click Select Top Result for All Sets.
Click Add Selected Assays to Cart.
Choose options (e.g., synthesis scale, purification options, flourescent dyes, and quenchers), and follow screens to checkout.

Resources

Need help or inspiration?

If you need further assistance please call, email, or web chat us today.