Oligos incorporating locked nucleic acids (LNAs) for enhanced stability and nuclease resistance
Affinity Plus DNA & RNA Oligonucleotides are custom, single-stranded and duplexed sequences that contain 1–20 LNA nucleotides. Incorporation of the LNA nucleotides confers increased target specificity and oligo stability. Including Affinity Plus LNA nucleotides in your oligos:
See the Locked nucleic acids (LNA) technology page for the structure of these modified bases and to learn about the many applications they can facilitate.
The following annealing fees will be applied to each duplex ordered:
Annealing fees will be applied to duplexed oligos (per duplex), as follows:
Shipped dry, or resuspended to your specifications. A minimum of 24 and 96 oligos required for 96- and 384-well plates, respectively.
1 oligo pair per well, annealed. Shipped dry. A minimum of 24 and 96 oligo duplexes are required for 96- and 384-well plates, respectively.
Shipped dry or resuspended to your specifications. A minimum of 24 or 96 oligos required for 96- or 384-well plates, respectively.
Affinity Plus DNA & RNA Oligonucleotides are LNA single- and double-stranded sequences. With the Oligo Entry ordering tool, you can design the sequence you require with 1 to 20 LNA nucleotides to suit your specific research needs.
Every Affinity Plus DNA & RNA Oligo you receive is deprotected and desalted to remove small molecule impurities. In addition, your oligos will be
*With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
To enter individual Affinity Plus LNA nucleotides, insert "+" before the base (+A, +C, +G, +T).
Affinity Plus Oligonucleotides provide identical annealing properties as other manufacturers’ LNA sequences (Figure 1). The increased hybridization affinity and thus hybridization melting temperature (Tm) means enhanced sequence stability both in vitro and in vivo.
The Affinity Plus LNA sequences can be used as probes, for example, in qPCR and genotyping assays. The higher Tm of these sequences provides better hybridization stability to target regions, especially those with low GC content. This increased stability also makes it possible to use shorter probe designs, which are helpful when target regions are limited in size. For these applications, obtain these LNA probes as Affinity Plus qPCR Probes. These LNA probes provide the same sensitive amplification as other LNA probes, and show increased sensitivity over unmodified probes (see Figure 2 on the Performance tab of the Affinity Plus qPCR Probes product page).