For demanding applications such as multiplex PCR, cloning, mutagenesis or antisense/RNAi methods, additional purification can significantly improve oligonucleotide performance. Purified oligos up to 60 bases that are provided with a purity guarantee receive
QC by capillary electrophoresis (CE). The traces are available online in your account order history.
Purification services offered include PAGE and various types of HPLC. Purified, unmodified oligos 20–50 bases in length are provided with the guaranteed yields (Table 1).*
Table 1. Yield guarantees for unmodified oligos of 25–50 bases, after purification.
|Purification type||100 nmol||250 nmol||1 µmol||5 µmol||10 µmol|
|PAGE||2 nmol||6.5 nmol||25 nmol||125 nmol||250 nmol|
|HPLC||3.75 nmol||12.5 nmol||50 nmol||250 nmol||500 nmol|
|IE-HPLC||3.75 nmol||12.5 nmol||50 nmol||250 nmol||500 nmol|
|RNase-Free HPLC||3.75 nmol||12.5 nmol||50 nmol||250 nmol||500 nmol|
|Dual HPLC||2 nmol||6.5 nmol||25 nmol||125 nmol||250 nmol|
|Dual PAGE & HPLC||0.5 nmol||3 nmol||12.5 nmol||62.5 nmol||125 nmol|