The xGen™ Hybridization and Wash Kit has been designed for use with xGen Hyb Panels and xGen Universal Blockers. The kit is compatible with the protocol, xGen hybridization capture of DNA libraries for NGS target enrichment, to provide
a complete, high-quality hybridization capture solution for targeted NGS research.
Prevent adapter cross-hybridization by using xGen Universal Blockers in your hybridization capture reactions. Adapter sequences are ligated to all library fragments, both on-target and off-target, before enrichment. These adapter sequences can hybridize
with each other during enrichment, creating a "daisy-chain" effect, with off-target fragments being captured alongside on-target fragments, which can impact sequencing efficiency.
xGen Universal Blockers bind to platform adapter sequences on a designated strand (usually the inverse of the synthetic adapter) to help prevent non-specific binding. Blocking the adapter sequences significantly improves capture results [1,2].
We have partnered with several instrument vendors to develop automation scripts for research using the xGen hybridization capture protocol on liquid handling robots. Scripts are currently available for the following:
- Beckman Coulter Biomek™ i5 and i7 Automated Workstations
- Hamilton NGS STAR™ for Library Prep Workstation
- Perkin Elmer Sciclone™ Workstation
Please contact the instrument vendors for more information regarding script availability and installation or contact IDT Customer Care for an updated list of qualified automation scripts for liquid handling