The xGen Normalase Module offers a novel enzymatic library normalization technology that consolidates DNA or RNA library normalization and pooling for loading on Illumina® systems in research studies. The Normalase workflow
eliminates the need for library concentration adjustment prior to library pooling, improving cluster density and library balance.
The xGen normalization method can easily be integrated into standard library preparation protocols to improve turnaround
time and loading accuracy for NGS laboratories. The library selection and enzymatic normalization steps of the Normalase workflow (Figure 1) are designed for research workflows that consistently produce amplified library yields 3x the target normalization
amount following library amplification with Normalase primers. For example, ≥6 nM or ≥12 nM yield in 20 μl volume achieves 2 nM or 4 nM normalized library yield; a ≥6 nM normalization workflow will result in a 2 nM final normalized library
pool, which can be concentrated to achieve 4 nM.
This workflow does not introduce a second PCR; instead, it replaces the primers in conventional library amplification, either terminal or indexing. The xGen Normalase Kits offer a fast, scalable
library normalization workflow for high-throughput research laboratories.