Complete coverage of the human mitochondrial genome
The xGen Human Mitochondrial DNA (mtDNA) Hyb Panel is designed to simplify the investigation of human mitochondrial genes by delivering optimal coverage across the entire >16 kb human mitochondrial genome in research samples.
xGen NGS—made for human mtDNA research.
For the xGen Hybridization and Wash Kit, xGen Universal Blockers, xGen Library Amplification Primer Mix, and/or xGen Human Cot DNA, please visit the xGen Hybridization Capture Core Reagents page.
The xGen Human Mitochondrial DNA (mtDNA) Hyb Panel provides coverage of the human mitochondrial genome in research studies. The panel consists of 138 individually-synthesized xGen Hyb Probes and delivers reliable coverage of the entire >16 kb mitochondrial genome.
This flexible, modular panel can be used as either a standalone† or spike-in panel, for cost-effective sequencing of variants in mitochondrial DNA in research samples. When added to other xGen Hyb Panels, the xGen Human mtDNA Hyb Panel does not
affect the high target specificity or coverage uniformity of the original panel.
†Because the mitochondrial genome comprises a small target space, the xGen Human mtDNA Hyb Panel contains relatively few probes compared to other target capture panels. When used as a standalone panel, expect lower on-target results than when used as a spike-in to a larger panel. This is typical of small panels in hybridization capture enrichment.
Figure 1. An example showing the spike in of the xGen Human mtDNA Hyb Panel does not impact on-target rate of recipient panel. DNA libraries were prepared from 100 ng of NA12878 genomic DNA (Coriell). 500 ng of the libraries were enriched with a custom panel comprised of 509 xGen Hyb Probes, with (n=8) and without spike in (n=3) of the xGen mtDNA Hyb Panel. The enriched libraries were sequenced on a MiSeq® System (Illumina) using 2 x 150 paired-end reads. Each sample was sub-sampled to 0.5M total reads. The averages of on-target values (with 150 bp flank) across experiments are shown.
Figure 2. Example of uniform coverage of targeted regions using xGen Human mtDNA Hyb Panel. Genomic DNA libraries were enriched using the human mtDNA Panel spiked into a custom panel containing 509 probes (n=8). Enriched samples were sequenced on a MiSeq system using 2 x 150 paired-end reads. Total reads for each sample were sub-sampled to 0.5M total reads. The average normalized target coverage ranged between 0.56X and 1.31X for the regions targeted by the mtDNA Panel. Normalized target coverage bounds at 0.5X and 1.5X are denoted by the doted lines.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.