More posts in DECODED

• Calculations: Converting from nanograms to copy number
• What is CRISPR screening?
• Comprehensive genomic profiling—what, why, and its future
• Oligo synthesis: Why IDT is a leader in the oligo industry
• Double-quenched probes increase signal to noise ratios by decreasing background fluorescence
• Unraveling RNA—The importance of a 2' hydroxyl
• What you need to know about gene fusions
• What is a phosphodiester bond?
• Troubleshooting your site-directed mutagenesis by PCR
• Genome editing tip: A CRISPR RNA annealing step can increase editing efficiency
• Amplicons and amplicon sequencing
• Cloning strategies: Blunt-end cloning
• Mechanical vs. enzymatic fragmentation approaches: Which method best improves library quality?
• Consider ribonucleoprotein (RNP) instead of CRISPR plasmids!
• Beginner’s guide to next generation sequencing
• CRISPR-Cas nucleases: Understanding PAM requirements and Cas diversification strategies
• Understanding the importance of 3’ UTR mutations in cancer research
• How DNA ligation works
• Luciferase, an alternative to fluorescence
• How to use CRISPR-Cas9 technology to generate mice models for research

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