The rhAmpSeq CRISPR Analysis Tool is capable of singleplex and multiplex analyses.
In singleplex analysis, the sample library has only one amplicon that is used to assess the editing of a single site in a sample. You would use singleplex analysis to screen numerous guide RNAs for on-target activity.
In multiplex analysis, the sample library is generated by multiplex PCR and is composed of multiple amplicons that assess the editing on multiple sites in a sample. You would use multiplex analysis to assess the on- and off-target editing of a guide RNA.