Flexibility to meet a wide range of guide RNA needs
Alt -R CRISPR Custom Guide RNAs are chemically synthesized and modified. These guide RNAs include pegRNA for the prime editing system as well as guide RNAs for the Cas13 family of enzymes and other unusual Cas systems.
Prime editing guide RNA (pegRNA)
The laboratory of David Liu (Broad Institute of MIT and Harvard, Cambridge, MA) has published a CRISPR genome editing technique called prime editing .
This utilizes a Cas9 H840A nickase fused to a reverse transcriptase, in combination with a long guide RNA (prime editing guide RNA or pegRNA). The pegRNA encodes the new sequence and allows transcription to introduce the desired mutations. This system
may have great promise for research geared towards the development of new therapies for genetic diseases. It is an exciting complement to existing CRISPR editing systems and may even be an improvement in many cases.
Cas13 guide RNA
The CRISPR-Cas13 family of enzymes targets and cuts RNA instead of DNA. Papers from the Feng Zhang lab at the Broad Institute and the Stanley Qi lab at Stanford University demonstrate the potential of CRISPR-Cas13 for SARS-CoV-2 identification as well
as the research studies geared towards the development of COVID-19 therapies. [2,3]