Amplicon sequencing is a type of targeted next generation sequencing that uses PCR to create sequences of DNA called amplicons. It is simple and fast and has a variety of applications.
This detailed overview walks you through major advances in capture and enrichment technology, types of targeted next generation sequencing, their applications, and more.
Amplicon sequencing is a quick and easy method of targeted sequencing with a simple workflow and fast turnaround time. Amplicon sequencing has naturally higher on-target rates than other targeted sequencing methods due to the specificity of primer design.
This method is appropriate for genotyping by sequencing, detection of germline single nucleotide polymorphisms (SNPs), insertions/deletions (indels), and known fusions. Amplicon sequencing should be used for experiments that require less sensitivity, meaning they can tolerate more false positive variant calls, since bias that might be created during the PCR amplification step cannot be removed.
IDT has developed 2 unique, high-performing targeted NGS approaches to maximize researchers’ ability to confidently identify and confirm biomarkers in germline and somatic mutations. Here, we describe the benefits and caveats of using either hybridization capture or amplicon sequencing to identify mutations in normal tissue, formalin-fixed, paraffin-embedded (FFPE) tumor, and cell-free DNA (cfDNA).
Working in one of these applications? Just starting? See how you can easily improve your workflows and results.
The rhAmpSeq™ system enables highly accurate amplicon sequencing on Illumina® next generation sequencing (NGS) platforms. Whether you are investigating thousands of targets or a few, the fast and easy rhAmpSeq workflow generates NGS-ready amplicon libraries for deep, targeted resequencing.
Learn how other scientists have applied amplicon sequencing technology in the field.
Read our articles about the rhAmpSeq amplicon sequencing system.