Your product is now available from Integrated DNA Technologies.
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Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
How are IDT HDR donor oligos modified?
We provide 3 options:
Alt-R HDR modification. This is our recommended option for the best HDR performance. It includes a proprietary modification pattern developed through extensive testing to increase donor oligo stability and enable the highest rate of repair.
Phosphorothioate (PS) bond modification. There are 4 PS bonds total; the first 3 and last 3 bases of the HDR donor oligo incorporate these bonds in the sequence.
Example: N*N*NNNN…NNNN*N*N (* = phosphorothioate bond; N = DNA base)