Integrated DNA Technologies acquires Archer™ next generation sequencing research assays to advance actionable scientific discoveries.

Order by stock part number »
Get Help
 |  EN
Sign In
ProcessingProcessing
  1. Products
  2. qPCR & PCR
  3. Master mixes & reagents
21_NG_ProductOverview_HeaderIcon_qPCR

qPCR and PCR master mixes, enzymes and reagents

Using commercially produced master mixes, enzymes, and reagents ensures simple, efficient experiment setup and consistent data.

PrimeTime™ Gene Expression Master Mix

PrimeTime Gene Expression Master Mix is a hot-start master mix delivering high quality results under either conventional or fast cycling conditions. Optimized for probe-based qPCR.

Learn more

PrimeTime™ One-Step RT-qPCR Master Mix

The PrimeTime One-Step RT-qPCR Master Mix is a ready-to-use, 2X concentrated mixture that is designed for use in probe-based, real-time quantitative PCR. It contains an antibody-mediated hot-start DNA polymerase, reverse transcriptase, dNTPs, MgCl2, enhancers, and stabilizers. PrimeTime One-Step RT-qPCR Master Mix offers high quality results for routine one-step qPCR experiments.

Learn more

PrimeTime™ One-Step 4X Broad-Range Master Mix

PrimeTime One-Step 4X Broad-Range Master Mix is a ready-to-use 4X concentrated mixture that is designed for use in probe-based, real-time quantitative PCR. It is a premium one-step qPCR master mix that offers advanced capabilities including the option to amplify from crude or purified samples. It contains a hot-start reverse transcriptase, hot-start DNA polymerase, dNTPs, buffer components, and an enhancer solution which improves direct amplification from difficult samples.

Learn more

rhAmp™ Genotyping Master Mix and Reporter Mixes

rhAmp Genotyping Master Mix and rhAmp Reporter mixes are specially formulated for use with rhAmp SNP Assays to deliver enhanced allelic discrimination and signal generation. Synthetic control templates are available during the integrated ordering process.

Learn more

RNase H2 enzyme

Increase the precision of target sequence amplification by limiting negative effects from primer-dimers. For use in customer-developed rhPCR assay applications, such as rare variant identification, splice variant discovery, or microbial identification studies.

Learn more

Water, buffer and nuclease control

Buffers, water, and work surfaces free of nucleases are important to ensure sample integrity and accurate results when performing qPCR experiments. Use IDTE, Nuclease-Free Water, and Nuclease Decontamination Solution to provide a clean environment for qPCR studies.

Learn more

Overview

Using commercially produced master mixes, enzymes, and reagents ensures simple, efficient experiment setup and consistent data. Each batch of reagents from IDT undergoes rigorous quality assessments for performance and reproducibility, moving you one step closer to achieving successful and consistent qPCR results.

Mastermix2-step RT-qPCR1-step RT-qPCRDirect amplification from crude samples2X4XInhibitor resistantShipping condition
PrimeTime Gene Expression Master Mix✔  ✔ ✔Ambient temperature
PrimeTime One-Step RT-qPCR Master Mix ✔ ✔ ✔Dry ice
PrimeTime One-Step 4X Broad-Range Master Mix ✔✔ ✔✔✔Dry ice

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

RUO22-1043_001