Exome sequencing sequences only the protein-coding regions of the genome. Since exons represent only 1% of the human genome, an effective method of separating these regions from noncoding DNA is critical to focus on potentially important mutations implicated
in disease. The captured material must also be suitable for sequencing to a satisfactory depth of coverage for reliable detection of variant alleles and sensitive applications like copy number variation (CNV) detection. To provide increased depth
of coverage and enable high multiplexing of samples, the xGen Exome Research Panel v2 targets only the coding sequences (CDS) of human coding genes in the RefSeq 109 database.
xGen Exome Research Panel v2 consists of 5′ biotin–modified oligonucleotide probes that are individually synthesized and individually analyzed by ESI-MS and optical density (OD) measurement. The probes are normalized before pooling to ensure
that each probe is represented in the panel at the correct concentration. Probes that fail quality control are resynthesized. This rigorous manufacturing process gives xGen Exome Research Panel v2 a unique advantage over array-derived pools in which
missing or truncated probes cannot be identified before sequencing. IDT proprietary synthesis methods enable challenging probes, such as those with high GC and AT content, to be appropriately represented in the panel. The individual probes are synthesized
on a larger scale than that which is available for array manufacturing, allowing IDT to produce a single, large lot of material that is aliquoted over time, providing consistent results for years.
xGen Exome Research Panel v2 consists of 415,115 individually synthesized and quality controlled xGen Lockdown Probes. The panel spans a 34 Mb target region (19,433 genes) of the human genome and covers 39 Mb of probe space, the genomic regions covered
by probes. Probes were designed using our new “capture-aware” algorithm and assessed with proprietary off-target analysis, ensuring the most complete design coverage. All probes in the panel are manufactured under strict ISO 13485 standards.
Mass spectrometry and dual quantification measurements of each probe ensure the quality of the probe and its appropriate representation in the pool.