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Technical reports

Comprehensive discussions of nucleic acids and their properties, calculations, strategies, techniques, and applications.

For overviews of common biotechnology concepts and methods, see our biotech basics.

PDF tag gBlocks gene fragments as qPCR standards (361 KB)
PDF tag gBlocks gene fragments as qPCR standards (361 KB)
PDF tag Troubleshooting polyacrylamide gel electrophoresis (PAGE) (442 KB)
PDF tag Troubleshooting polyacrylamide gel electrophoresis (PAGE) (442 KB)
PDF tag In vitro nuclease detection (416 KB)
PDF tag In vitro nuclease detection (416 KB)
PDF tag Fluorescence and applications of fluorescent oligonucleotides (703 KB)
PDF tag Fluorescence and applications of fluorescent oligonucleotides (703 KB)
PDF tag Fluorescence quenching by proximal G bases (202 KB)
PDF tag Fluorescence quenching by proximal G bases (202 KB)
PDF tag Spectra of DNA-conjugated fluorescent dyes (228 KB)
PDF tag Spectra of DNA-conjugated fluorescent dyes (228 KB)
PDF tag Introducing antisense oligonucleotides into cells (420 KB)
PDF tag Introducing antisense oligonucleotides into cells (420 KB)
PDF tag RNA interference and DsiRNAs (249 KB)
PDF tag RNA interference and DsiRNAs (249 KB)
PDF tag Dicer design rules for RNAi (153 KB)
PDF tag Dicer design rules for RNAi (153 KB)
PDF tag Identification of antisense oligonucleotide motifs (240 KB)
PDF tag Identification of antisense oligonucleotide motifs (240 KB)
PDF tag Designing antisense oligonucleotides (386 KB)
PDF tag Designing antisense oligonucleotides (386 KB)
PDF tag Strategies for attaching oligonucleotides to solid supports (667 KB)
PDF tag Strategies for attaching oligonucleotides to solid supports (667 KB)
PDF tag Ultramer Oligonucleotides (519 KB)
PDF tag Ultramer Oligonucleotides (519 KB)
PDF tag Quantification of oligonucleotides with NanoDrop instruments (664 KB)
PDF tag Quantification of oligonucleotides with NanoDrop instruments (664 KB)
PDF tag Calculating Tm for oligonucleotide duplexes (215 KB)
PDF tag Calculating Tm for oligonucleotide duplexes (215 KB)
PDF tag Chemical synthesis of oligonucleotides (540 KB)
PDF tag Chemical synthesis of oligonucleotides (540 KB)
PDF tag Stability of oligonucleotides (639 KB)
PDF tag Stability of oligonucleotides (639 KB)
PDF tag Mass spectrometry analysis of oligonucleotides (383 KB)
PDF tag Mass spectrometry analysis of oligonucleotides (383 KB)
PDF tag Capillary electrophoresis of oligonucleotides (418 KB)
PDF tag Capillary electrophoresis of oligonucleotides (418 KB)
PDF tag Breaking PCR (337 KB)
PDF tag Breaking PCR (337 KB)